Cannabis growing – Asexual Propagation. Part 1
Asexual propagation ( cloning ) permits the protection of genotype because only ordinary cell division ( mitosis ) happens during expansion and regeneration. The vegetative ( non-reproductive ) tissue of cannabis has ten pairs of chromosomes in the nucleus of each cell. This is sometimes known as the diploid ( 2n ) condition where 2n = twenty chromosomes. During mitosis each chromosome pair copies and one of the 2 matching sets of chromosome pairs migrates to each child cell, which now has a genotype matching to the mummy cell. Therefore , each vegetative cell in a cannabis plant has the same genotype and a plant springing from asexual propagation will have the same genotype as the mum plant and will, for all practical purposes, develop identically under the same environmental conditions. In Weed , mitosis occurs in the shoot peak ( meristem ), root tip meristems, and the meristematic cambium layer of the stalk. A propagator gets use out of these meristematic areas to provide clones that may grow and be multiplied. Asexual propagation methodologies like cuttage, layerage, and division of roots can guarantee matching populations as big as the expansion and development of the parental material will permit.
Clones can be produced from even a single cell, because each cell of the plant possesses the genetic info required to regenerate a total plant. Asexual propagation produces clones which continue the unique traits of the parent plant. Due to the heterozygous nature of cannabis , valuable characteristics could be lost by sexual propagation that may be saved and multiplied by cloning. Propagation of almost matching populations of all-pistillate, fast-growing, uniformly maturing cannabis is made viable thru cloning. Any rural or environmental influences will affect all the members of that clone similarly. The theorem of clone does not always mean that all members of the clone will always appear identical in all traits. The phenotype that we observe in an individual is influenced by its environment. members of the clone will develop differently under varying environmental conditions. These influences don’t affect genotype and so aren’t permanent. Cloning in prinicple can pre serve a genotype for all time. Spirit may slowly decline due to poor choice of clone material or the recurring pressure of illness or environmental stress, but this trend will re verse if the pressures are removed. Shifts in genetic composition now and then happen during selection for powerful expansion.
If parental strains are maintained by in frequent cloning this is less certain. Only mutation of a gene in a vegetative cell that then divides and passes on the mutated gene will permanently affect the genotype of the clone. If this mutated portion is cloned or reproduced sexually, the mutant genotype will be further duplicated. Mutations in clones sometimes affect dominance relations and are thus spotted straight away. Mutations could be prompted artificially ( but without much predictability level ) by treating meristematic regions with X-rays, colchicine, or other mutagens. The genetic uniformity offered by clones offers a control for experiments engineered to quantify the sophisticated results of environment and cultural systems. These subtleties are often obscured by the intense diversity caused by sexual propagation. clonal uniformity can also invite major problems…
If a population of clones is the subject of unexpected environmental stress, pests, or disease for which it has no defense, each member of the clone is certain to be influenced and the whole population could be lost. Since no genetic variety is located in the clone, no adaption to new pressures can happen thru recombination of genes as in a sexually propagated population. In propagation by cuttage or layerage it’s just obligatory for a new root system to form, since the meristematic shoot apex comes right from the parental plant. Many stem cells, even in mature plants, have the capacity of making fortuitous roots. In reality each vegetative cell in the plant contains the genetic info needed for a complete plant. Fortuitous roots appear casually from stems and old roots vs endemic roots which appear along the developing root system originating in the embryo. In wet conditions ( as in the tropics or a green house ) fortuitous roots happen naturally along the primary stalk close to the ground and along limbs where they sag and touch the ground. Rooting. An awareness of the internal structure of the stem is useful in understanding the origin of fortuitous roots.
The development of fortuitous roots can be broken down into 3 stages :
1) the initiation of meristematic cells found just outside and between the vascular bundles ( the root initials ),
2) the differentiation of these meristematic cells into root primordia, and
3) the emergence and expansion of new roots by bursting old stem tissue and building vascular connections with the shoot.

